To help ensure that necessary
parameters have been set, AutoQuant does require that datasets be loaded into
the software in order to be processed. However, there are a few ways to help
expedite the process when working with hundreds or even thousands of stacks.
Assuming that your data is stored
such that each file represents a stack or otherwise complete dataset (as
opposed to having each file represent one frame of a dataset, for example), then
my first suggestion is to open a Windows Explorer to the folder containing your
stacks, then drag them all directly to the batch window at once, as shown here:
From AutoQuant X 3.0.4 onward, the collection of files will
be loaded into the batch in the background, allowing you to set up the batch
using the first entry while the remaining entries load.
Start by selecting the task (e.g., 3D Deconvolution). If the
“Valid?” column switches to “Yes” after that, then the task could launch as-is.
If it continues to display, “No”, then click on the word “No” to open the
deconvolution dialog to see what was flagged as invalid.
Once the sets have completed loading, and once the first
task has been set up, then you can copy whatever settings you need and launch
the batched tasks in just a few clicks. Start by right-clicking on the row that’s
been set up.
You’ll notice the menu has some copy/paste options that are
divided into a couple of categories: optics settings and operational
settings. The “all settings” entries cover both categories.
Next, you’ll want to paste your settings to the remaining
rows. I recommend including the row you’re copying from in your selection.
It doesn’t hurt anything, and it makes it easier to launch everything in fewer clicks.
Also, as a tip, when you select rows in the batch window –
whether it be just one, or multiple rows – click in the Row Header area of the appropriate row(s), as indicated below. By
interacting with that area instead of the Row
Data area, it is possible to use selection shortcuts like Click+Drag, Ctrl+Click,
and Shift+Click. The latter – selecting the first row, scrolling to the bottom,
then Shift-clicking the last row – is my preference for working with large
numbers of sets.
When all of the rows are selected, right-click in the Row Data area of any row in the
selected region, and choose a paste option. You can skip some waiting and some
clicking interaction by choosing one of the “compound” options at the bottom, like
“Paste Operational Settings and Launch” if you didn’t have to change any optics
settings, or “Paste All Settings and Launch” if you did.
I hope that helps streamline your workflow!