YuriG

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YuriG
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  • What do you want to measure? Looking at the image, I can guess, you can measure diameter of the cell (segmenting using Smart segmentation): Or area of the cell with all outgrowth (after Variance filter): Yuri
  • inexperienced, Getting intensity measurements from multiple ROIs in Image-Pro Premier is much easier than in IPPlus. We have a special tool "Intensity Tracking" (Measure tab) specifically for that (you don't need a macro). You just draw measurement…
  • Matt, You can get path to Configuration Files folder using this line: ThisApplication.Path(mcPathType.mcptConfigurationFiles) Yuri
  • Matt, The default, persistable SpatialCalibrations.icq file is located in the Configuration Files folder. You may have other ICQ files in any location on the computer, but they will not be loaded automatically. Yuri
  • Yes, it's possible to set track color. Here is the modified macro that adds White reference track: Public Function AddMaxTrack() As SimpleScript AddMaxTrack = New SimpleScript Dim trdoc1, obj1 'define the range of the…
  • The orientation plot is auto-scaled to the extends of the existing tracks, so it's not possible to define the axis ranges. As a workaround, you can add a track that would define the max extends of the displacement. Here is the macro that will add a…
  • Kevin, If you use the same illumination conditions and stable light source, and you just need to compare results between different experiments, then you can do measurements without intensity calibration. In that case the values are just pixel gray …
  • WesanSyu, The FeatureFromPoint is for low level functions. If you use Count/Size then you should use higher level functions, below is the macro that hides corner objects: Public Sub GetBoundingBoxes() Dim im As McImage=ThisApplication.…
  • WesanSyu, If you want to hide a measurement object in Image-Pro 10, the easiest way would be to select this object (using an interactive macro command) and then delete or hide it.(the commands are recordable). Yuri
  • Matt, Yes, this tool doesn't handle rotation and scale, just translation as it's based on FFT cross-correlation.  Yuri
  • Hi Vinay, If you want to segment and measure all objects, then you can use the workflow described by Matt. But if you just need a count of objects of certain shape, then you can use Find Shapes tools located on the Extras tab (the tab should be ena…
  • Yes, it is possible. You just need to hide unwanted objects using one of the available methods:    1. click "Show Selected" button in Data Table toolbar (or in the Count/Size ribbon, Select group, drop-down) or    2. click "Show Class 1" (use the n…
  • It is also possible to put all class stats in one row, you just have to add custom measurements moving Class stats to Measurements Stats table. I've attached the ADOXSD file, which will collect Sum of areas per class into 3 different columns, so it …
  • What you can use is the Measurement Class Table and Area(Sum) measurement in the Data Collector Then you just collect the data and get the singe value per class. I've also attached the Data Collector measurements file (zipped). Regards, Yuri :
  • Hi Carlotta, Both tools "Count by ROI" and "Single Variable Classification" use "Classes" to separate objects, so if you execute Classification after Count by ROI the existing classes are overwritten by Classification classes. There is also another…
  • Juan, Regarding binary mask: Click the Binary Mask button, in the Threshold Tool dialog set the right threshold (e.g. 150) and click the Mask button - it will create a new binary sequence.  Yuri
  • Juan, I don't see the calibration of your image, but the steps on the graph may be just 1 pixel, so you cannot go smoother (unless you smooth the graph itself). Regarding detecting at fixed threshold of 150: you can use Threshold tool to make a ma…
  • Ok, I can see that you are using Interaction command. In my example above I showed how to use interaction in the Thresholds command, here is how the code should look in Interaction command: With Automate.ScriptingCommands.Interaction(Adjust…
  • The project explorer comes up when the input of the interactive command is Nothing. You can set the input to doc1(your active image) or even a panel. Here is an example how to show prompt on the left side of the Threshold Tool panel: With M…
  • Here is a more complex macro that will apply new LUT to color composite view: Public Function ApplyToCC() As SimpleScript ApplyToCC = New SimpleScript With Automate.ScriptingCommands.CodeCommand(ApplyToCC) If .R…
  • Do you mean that it's not always working for "15' HGF LAMP488 4.lsm" image (in Colocalization folder)? Or it's working for the demo image, but it's not working for your own image? Yuri
  • fsup,  Do you need the whole image or just some area of it? If you need an area, I recommend to put a ROI around that area (on the original VLI image) and Extract that area  (at the desired resolution). It will be a fast operation, which is also re…
  • Hi fsup, You should be able to record such macro just by switching off color composite mode and selecting the channel. Here how it looks in my recording, the macro selects the channel 2 image (0-based): Public Function ActivateChannel2() As Si…
  • Important point is to have always the same starting conditions, in your case the 1st channel should be active when ColorComposite gets switched off (otherwise you need to record activation of the first channel). If it still doesn't work, try to repr…
  • I checked your macro and can see the problem. The issue is that the composite view is updated after some delay (when all messages are processed), so in the macro the view is exported to the PowerPoint before it gets updated. Adding some delay before…
  • Kristi, If you want to measure spots o different color, then you can use Smart segmentation and get results as on my screenshot, measurement options attached. If you want to measure skin roughness, then you can apply some Edge filter (e.g. Edge M…
  • I've just attached it to the first answer.
  • If you need just 3 intensity bins, you don't need Count, you can get these values directly from intensity histogram (much faster). I've attached the app that makes it easier: define 3 ranges in the Threshold dialog and it will report you area percen…
  • Dan, I don't see this problem. If I count Spots.tif (Auto-dark) the Heterogeneity values are in range from 0.17 to 0.41. Can you post the test image and the IQO file with segmentation parameters? Thanks, Yuri
  • Kristi, It's difficult to recommend something without seeing the image. Can you post some screenshots? Yuri