Image size

BTP

Hi, I am struggling with how to know the size of the image I am taking, I am trying to count the number of objects within an image and to create a 'concentration' by knowing what space they occupy. Do I need to draw a line over the image in horizontal and vertical directions? Any help would be greatly appreciated.

YuriG

Hi BTP,

First you need to calibrate the image: just capture an image with a micrometer and create calibration. The easiest way is to use "Quick calibration" (Capture tab, Calibration group, Create dropdown). There is also "Auto-calibration" option that works on images with micrometer. (more details you can find in Help)

Then you just draw measurement lines (Measure tab, Direct group) and the length will be reported in the data table. You can also make the length to be shown on the image, if you set measurement label text to "Measurement" (Measurement options panel).

Also, there are many other ways to get image area besides drawing lines.

Regards,

Yuri

YuriG

If you are using Count/Size, then you can use "Percent Area (%)" measurement to find the area percentage of the object to whole image (or ROI) area.

Yuri

mbatchelor

2020-02-07-125032

BTP --

If you are working with a MACRO and you calculating

    CONCENTRATION = COUNT / AREA

Then:

1. Get the COUNT
2. Get the IMAGE AREA
3. Divide COUNT by IMAGE AREA to get CONCENTRATION

Get the COUNT with something like

                'CONNECT WITH THE AREA MEASUREMENTS IN THE IMAGE
                Dim My_Measure1 As New MeasEntry
                My_Measure1.Measurement = _
                    eMeasures.RgnArea

                'LEARN THE STATISTICS FOR THE OBJECTS IN THE IMAGE
                Dim My_Stats1(9) As Double
                My_Data1.CalcStatistics(My_Measure1,My_Stats1)

                '0 -- mcStatsMean -- mean value
                '1 -- mcStatsStDev -- standard deviation
                '2 -- mcStatsMin -- minimum value
                '3 -- mcStatsMax -- maximum value
                '4 -- mcStatsRange -- range
                '5 -- mcStatsSum -- sum
                '6 -- mcStatsIMin -- index Of minimum
                '7 -- mcStatsIMax -- index Of maximum
                '8 -- mcStatsNItems -- number Of items

                'UPDATE THE DIALOG BOX

                textBox_BackgroundCount.Text =
                    Format(My_Stats1(8),"#,##0")

Get the IMAGE AREA with something like

        'Determine the IMAGE WIDTH and IMAGE HEIGHT
        Dim MyImageWidth As Integer = _
            ThisApplication.Activeimage.Width
        Dim MyImageHeight As Integer = _
            ThisApplication.Activeimage.Height

and determine the CALIBRATION INFORMATION with something like

        'Learn the SPATIAL CALIBRATION INFO for this IMAGE
        Dim MyTempCalibUnits As String
        Dim MyTempCalibPixelSize As Single

                MyTempCalibUnits = _
                    ThisApplication.ActiveImage.SpatialCalibration.UnitAbbrev
                MyTempCalibPixelSize = _
                    ThisApplication.ActiveImage.SpatialCalibration.PixelSizeX

Now you can figure the CALIBRATED IMAGE AREA and then you can divide that value into the COUNT to get your concentration.

I hope this information is helpful.

-- Matt

BTP

Thanks Both, unfortunately Matt I have no experience of macros so will be unable to follow these instructions.
I don't quite want to do the calculation in the software but am very keen to know if the software will allow me to draw a line and know the length of that line. I have already calibrated the microscope with a stage micrometer, is there a way to simply see these line lengths within the software?

mbatchelor

2020-02-10-114253

BTP --

It sounds like some tailored training and perhaps just a bit of macro programming would be very beneficial to you.

Please contact me if you would like a quotation for time to perform this for you.

My contact info is below.

I hope this information is helpful.

-- Matt

Matthew M. Batchelor
President and Applications Engineer
Alces Imaging and Automation, LLC
Office: 281-646-7477