Make EDF at each timepoint.
I am working with LIF file which has time-lapse data for different positions of the well plate. At each position for each time point I also have z-stacks. I open one position time series (also with z-stacks) to apply the EDF , it seem it only creates the EDF from the time point that is active in the viewer. Is it possible to cycle through each data set (positions) and create EDF for each time point. It would be great if the result are retained as time serie.
Thanks for the test image. I checked it and found that Image-Pro does EDF on all time points only if the image set contains multiple channels. We will fix the problem in the next patch, so single-channel image sets will be processed as well.
As a workaround for the current version I've created a macro (attached) that will process all time points.
1. Open the attached project from the Apps tab. (or Project Explorer)
2. Open one of the wells with all time points from your LIF file.
3. Run "EDFAllTimePoints" macro from EDFImageSet project - it will create a new image set with EDF projections of every time point. (e.g. 23 time points image set)
4. Repeat steps 2-3 for every image set in LIF file.
Be sure you are using the latest version (10.0.7), it creates EDF for a complete set (all wells, all locations), the same for Maximum/Minimum functions (in case you need to create MIP image from Z-planes of every well).
When I make EDF, I only end up having a single image at the active timepoint. Do i have to select all the timepoints ! and how ?
Attached here is LIF file content information:
each position has 23 timepoint and variable z-stacks.
If your image set is opened as a multi-Z, Time series, then all time points supposed to be processed by EDF in one operation.
If it's not the case, can you please send me a representative test image, so I can look at it?
Click here to upload files.
You can click the Extract dimension button
and then save the result sequence as TIF (or in any other multi-frame format).