How to save a segmentation image mask after object filtration

Danielro

Hello to everybody!

I would like to know how to export images with measurement masks layout on top.

Basically, I am segmenting my images based on the RGB pick tool and I am defining 2 classes of objects (nuclei and immunohistochemistry signal). Then I do some area measurements, and I am also filtering my objects based on area, so that I do not count areas smaller than 2 sq microns.

And I would like to export an image with the colored masks on top, after area filtration.

The only way I could find was to press “Create preview image” in the “Count and measure objects menu” after the actual segmentation. However, even though I come back to this menu after measuring the data and applying the filter, this preview image contains the un-filtered mask… Is it possible to obtain a mask of the full image (not a print screen) after applying the filter?

Thanks so much and all the best wishes from Romania!

Take care and stay healthy, you and your beloved ones!!!

Daniel

YuriG

Hi Daniel,

If you want to create a binary mask of segmented objects, after area filter, then use the Mask button on Count/Size tab.

If you want to make a screenshot of the image with mask, then use Snap button on the Review tab.

Regards,

Yuri

Danielro

Hi Yuri and thanks so much!
This solved my problem!
All the best thoughts,
Daniel