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Z-stack creation

I am looking for the process to z-stack a sample, the software guidance gives detail on how to process it later with EDF options. Is it as simple as taking the individual images and applying them together before using the EDF step to view all in focus? I am unable to see this in the software guide, could you point me in the right direction.


  • BTP,

    Yes, it is possible to acquire separate images with different focus (Z) positions and then create best-focus image using EDF. Multiple images can be opened as a sequence when you use "Open As Sequence" option in the file open dialog.

  • Thank you Yuri, I shall have a look at that

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