How can I count the number and area of larger darker cells compared to the area of smaller cells?

Braydenx

I have tried smart segmentation and then learning classification, but it does not pick up all of the larger darker cells, and when I get it to, it picks up too much of the background.

https://easyupload.io/m/cm38jl

YuriG

Hi Braydenx,

I checked your images. The staining of dark cells is very weak (espesially on 3502.1.16.a.tif image)), so I had to use a custom recipe (based on low-pass filtered I-channel of YIQ) to segment dark cells, which produced an acceptable result.
I've attached the segmentation options and Data Collector types that generate the measurements you need.

1. Load the test image
2. Load the attached segmentatoin options Segmentation2Classes.iqo and click Count. (both Dark and Light cells will be segmented)
3. On the review tab load the attached Data Collector types DataCollectorTypes2Classes.adoxsd and click Collect. (data will be collected to the Data Collector)

The results will be shown in the Data Collector table: Percent area of Dark and Light cells:



The Measurement Stats table contains custom measurement Dark Cells Pcnt that shows the area percentage of dark cells relatively to the the area of light cells:



You can use this recipe as starting point and tweak it, if necessary, for other images (click the Clear button in Recipe Options group and create new reference objects for 2 classes and background).

Yuri

mbatchelor

2021-11-30-171810

Braydenx --

I would like to see the EXAMPLE IMAGE that I believe that you have posted and attempt to answer your question. 

Unfortunately browsing to a link like the one that you posted has become a bad idea in this day and age of computer viruses and malware.

Will you please post your EXAMPLE IMAGE here in the FORUM?

Thanks.

-- Matt

Braydenx

Sorry. I uploaded it as an image rather than a file in my original post, and it didn't go through, I guess.

Braydenx

Thank you so much for your help! Where are the best places to learn about image pro without having to come to the forum every time I run into an issue?

YuriG

You can find more tutorials and webinars in our media library: https://www.mediacy.com/support/imagepro/media
Check our app center for source-code samples https://www.mediacy.com/appcenter/app-center/
And search this forum for specific questions.

Yuri