Particle Counting -- Counting Too Many/Counting Undesired Objects
Hello,
I have been using Image Pro Plus to count DAB stained positive cells. I am using the thresholding tool to isolate the desired cells. However, the software is picking up spots that are not the cells I want, as well as counting one cell as several signals. For example: in one crypt the software is recognizing 200 positive cells (when by eye I count 11). Is there a way I can fix this?
I have been using Image Pro Plus to count DAB stained positive cells. I am using the thresholding tool to isolate the desired cells. However, the software is picking up spots that are not the cells I want, as well as counting one cell as several signals. For example: in one crypt the software is recognizing 200 positive cells (when by eye I count 11). Is there a way I can fix this?
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