Determine Macrophages in image pro premier 9.1
Try to distinguish macrophages by surface area and by color (brown in my case). But not really close to what I want. Is there perhaps another solution for this? Attached a picture of a histology preparation - pig tonsil with an IHC stain.
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Have you tried Smart Segmentation?
I'm able to measure the area of cells stained brown using smart segmentation.
There's a slightly dated, but still helpful video here:
https://youtu.be/lI_URgYtJqM
Best Regards,
Andrew
It sounds as if you need to make a smart segmentation recipe to find nuclei, and then make a learning classifier to classify the positive and negatively nuclei.
You will see something like this:
Should you wish to use my measurement options (which I'm sure you can improve), they can be downloaded here:
https://mediacy.sharefile.com/d-sabae52e799ed47eda629c7af6b7c4251
Regards,
Andrew
Best Regards,
Ralph