Determine Macrophages in image pro premier 9.1

RSKok

Try to distinguish macrophages by surface area and by color (brown in my case). But not really close to what I want. Is there perhaps another solution for this? Attached a picture of a histology preparation - pig tonsil with an IHC stain. 

Andrew_B

Hi RSKok, 

Have you tried Smart Segmentation? 

I'm able to measure the area of cells stained brown using smart segmentation. 

There's a slightly dated, but still helpful video here:

https://youtu.be/lI_URgYtJqM

Best Regards,
Andrew

RSKok

Andrew_B said:

Hi RSKok, 

Have you tried Smart Segmentation? 

I'm able to measure the area of cells stained brown using smart segmentation. 

There's a slightly dated, but still helpful video here:

https://youtu.be/lI_URgYtJqM

Best Regards,
Andrew

Hi Andrew, thanks for reply. I know smart segmentation. but the tricky part is counting them. The macrophages does have a cell nucleus. I have also tried spit after. Thanks again for you answer.

Andrew_B

OK.  
It sounds as if you need to make a smart segmentation recipe to find nuclei, and then make a learning classifier to classify the positive and negatively nuclei. 

You will see something like this: 




Should you wish to use my measurement options (which I'm sure you can improve), they can be downloaded here:

https://mediacy.sharefile.com/d-sabae52e799ed47eda629c7af6b7c4251

Regards,
Andrew

RSKok

Hi Andrew thank you, i will give it a shot. It looks promising. 

Best Regards,
Ralph