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Determine Macrophages in image pro premier 9.1

Try to distinguish macrophages by surface area and by color (brown in my case). But not really close to what I want. Is there perhaps another solution for this? Attached a picture of a histology preparation - pig tonsil with an IHC stain. 
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Answers

  • Hi RSKok, 

    Have you tried Smart Segmentation? 

    I'm able to measure the area of cells stained brown using smart segmentation. 

    There's a slightly dated, but still helpful video here:

    https://youtu.be/lI_URgYtJqM

    Best Regards,
    Andrew



  • Andrew_B said:
    Hi RSKok, 

    Have you tried Smart Segmentation? 

    I'm able to measure the area of cells stained brown using smart segmentation. 

    There's a slightly dated, but still helpful video here:

    https://youtu.be/lI_URgYtJqM

    Best Regards,
    Andrew



    Hi Andrew, thanks for reply. I know smart segmentation. but the tricky part is counting them. The macrophages does have a cell nucleus. I have also tried spit after. Thanks again for you answer.
  • OK.  
    It sounds as if you need to make a smart segmentation recipe to find nuclei, and then make a learning classifier to classify the positive and negatively nuclei. 

    You will see something like this: 




    Should you wish to use my measurement options (which I'm sure you can improve), they can be downloaded here:

    https://mediacy.sharefile.com/d-sabae52e799ed47eda629c7af6b7c4251

    Regards,
    Andrew


  • edited July 2023
    Hi Andrew thank you, i will give it a shot. It looks promising. 

    Best Regards,
    Ralph
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