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autoquant decon batch error

I am trying to perform autoquant decon using batch selection. However, whenever I do the batch decon, the error shows that x, y and z calibration must be greater than zero, although I set these values in the spatial calibration section. Running the decon on individual image still works. 

Answers

  • Hi,
    I'm sorry you've run into an issue with the batch deconvolution. Using the data that you provided to my colleague, I looked into the issue, and found that there is indeed a bug when the images are basic TIFF stacks. We will address the issue in an upcoming patch. In the meantime, if you convert your data - or acquire it - into a format like OME TIFF, the batch should work properly.
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