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Apply Spatial Calibration from .lif file

mkozlomkozlo
in Image-Pro General Discussions
I have a lif file that was taken using a leica microscope and when I open it in imagepro there doesn't seem to be a way to view/apply the spatial calibration metadata. Is there a way to view/apply this metadata?

Answers

  • YuriGYuriG
    Hi mkozio,

    Image-Pro can read LIF files and its metadata, including calibration. You can check it with some open samples: https://zenodo.org/records/3382102#.Y57rFnbMJaQ

    If your image is opened without calibration, then, for some reasons, Image-Pro could not read the calibration. You can try opening this file in LAS software, ensure that the calibration is present, and then either resave it as LIF again or a TIF.

    Also, if you know calibration parameters (pix per unit), then you can create the calibration manually in Image-Pro and apply it to the image.

    Regards,

    Yuri

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