Making volume measurements in 3D constructor

ailask

I'm trying to determine the volume of a nucleus using the 3D constructor. I am able to generate the 3D image quite easily using my meta-data parameters from my ND2 file. Meta data being x=.129, y=.129 and z=.2 microns. However when I try to use the volume measurements option in the 3D constructor options I', not able to use the values of my metadata. I can only use integers which, distorts the volume measurement outputs that I get. Is there anyway to get around this? 

YuriG

When you load the volume to 3D Constructor you can specify Voxel size manually (if it's not loaded correctly automatically). I've attached the screenshot of the form.

Yuri

ailask

Yep I enter in my meta data here. But the second step when I am rendering an iso-surface for volume measurements it asks to specify x, y, and z again. And only allows integers.

YuriG

It's not size there, it's sub-sampling. Voxel size is defined by volume, so all your measurements should be correct.

ailask

So then I set the values for x, y, and z to 1 and this will not distort the measurements?

YuriG

No

ailask

When I put in 1,1,1 as the sub-sample it states my volume is 0. Attached screen shot.

YuriG

Hi ailask,

From your screenshot I can see that you haven't set the threshold properly (Surface level), it's on the maximum (117), so objects are not segmented. Move the level down and you will see proper objects with correct volume values.

Regards,

Yuri

ailask

Thank you Yuri that fixed the problem!