By default, Image-Pro Plus expresses
intensity measurements in terms of the actual pixel value contained in the image
bitmap. The Intensity Calibration command is used to express this value in other
terms. You would use this
command if you wanted to relate intensity to optical density, or relate
intensity to temperature or relate intensity to protein content, for
example.
The Intensity Calibration command is also used to establish
Black and Incident levels for optical
density experiments.
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The units are defined by Intensity Calibration. In most cases images don't have any intensity calibration, so the intensity is measured in gray levels. You can create a new intensity calibration, define your own units and assign it to image if necessary.
Answers
By default, Image-Pro Plus expresses intensity measurements in terms of the actual pixel value contained in the image bitmap. The Intensity Calibration command is used to express this value in other terms. You would use this command if you wanted to relate intensity to optical density, or relate intensity to temperature or relate intensity to protein content, for example.
The Intensity Calibration command is also used to establish Black and Incident levels for optical density experiments.
I will send you more information to your email address from your profile.