Thanks for your quick reply. I have no image processing
background, but learning myself for one of our study through image pro premier. Images are of grey scale not RGB and we are looking for optical spectrum and pixel intensity. Herewith I am describing image acquisition and sending off some of our images to
better assist us.
We takes multiple images of retina (which is the light sensitive
tissue lining the back of our eye) using con focal laser scanning retinal camera
at 30 degree field of view from each eye. The photographs are taken under two
(IR mode) ( Wavelength 780 nm) captured without any barrier filter
2) Auto fluorescence
mode: (Wavelength 488 nm) captured with barrier filter which blocks all waves
under 500 nm allows 500-560 nm
These photographs will be taken at pre (baseline) and post ingestion of oral florescent agent and then analyse for
Please see attached images from our camera for
It would be great if you could outline how we can study pixel and spectrum variation at each
point/white spotted areas on given images in terms of intensity and spectrum changes
and suggest ways to use some of image
pro premier tools for analysis.
Please let me know you need any further information.
Thanks very much