Problem with saving object data to ROI

Hi all,

Within my macros I have a czi image, extract channels, make grey scale image and rotate it.

I have a step for delineation and I save the drawn ROIs to roi file, automated with the original filetitle.

So I have an imagefile.czi and a imagefile.roi with two saved polygons - all fine.

Then I want to measure, so I do the same (extract, grey scale etc), reload the ROI (no problem) and measure (no problem).

However, if I want to save the measurement to the roi file, I get the error message 'Object ref. not set to instance of an object' as soon as I touch the Add button in the Features Manager...

Any idea why?

What I can say is that it's not an issue of a write protection of ROI file (was my first suspect), since also if I delete all, the same error occurs.

I attached a file with screenshots.

Many thanks

Daniel












Answers

  • Hi Daniel,

    Could you please attach your files (image and roi) to the post. If they are big, you can use any file sharing tools, like dropbox, etc.

    Thanks,
    Nikita.