Intensity/IOD measurements--"Sample?"

sf000

Hi everyone,

I was doing quantitative analysis using Integrated Optical Density for some Immunohistochemistry slides, and I'm not sure what "Sample" is calculating....I don't think it's number of cells because a lot of the cells were clumped together and unclear for counting. If anyone has experience, I would really appreciate the help. I'm using Image Pro Plus 7.0.

Thanks

For example, this is the data for one image:

CaSki Control
Stats	Area	Den./Inten. (	IOD	Den./Inten. (
Min	11	85.057732	1082	1082
(Obj.#)	61	87	61	61
Max	267	104.61111	25231.668	25231.666
(Obj.#)	88	49	88	88
Range	256	19.553383	24149.668	24149.666
Mean	60.860001	96.824051	5716.1602	5716.1602
Std.Dev	58.862553	4.8016558	5365.2544	5365.2544
Sum	3043	4841.2021	285808	285808
Samples	50	50	50	50

YuriG

"Samples" reports the number of separate objects. If your cells are clumped together, they are reported as 1 objects. You should use different threshold, some pre-processing or separation tools (manual, watershed,...) if you want to measure every cell separately.

Yuri