Right now I'm using FiberApp for thickness mesaurements. If I create a mask to exclude background, I'm not sure that the thickness mesaurement is realistic. Mask that appears after using dark/manual segmentation obviously depends on my decision where are the boundaries of an object, so I have problems how to standardise the procedure.I can do the mesaurements by clicking on the specific objects, but with a bunch of pictures there is just to much of handwork. I want to automatise a procedure a little bit more. I'm sending you an example of a picture I need to analyze. I want to know the thickness of DARK BLUE curves, without purple ones and without background. Pictures are really complex, so I would be really glad for any help I can get. Thank you in advance! A
first thank you for your quick response and your help. I'm not sure if I completely understand Line profile tool. So with this tool, I should draw few lines through the object where I want to mesaure thickness. Ok, but I still get just the information about the thickness of a specific line/curve from the picture. I would like to know the thickness of ALL the lines/curves in one picture, so that I can calculate average at the end. Is this possible with Line profile tool? I also want to know how to collect the data from this mesaurements? For example that the thickness is 5 mm and not in the form 2-7 mm. It would be easier to process the data in the end. Pseudo colored image shows just the layout of thickness throught the image and you can't really mesaure thickness from that, if I understand right?
Nevertheless, you gave me some ideas how to analyze the pictures. I managed to get rid of most of the background, just by using Smart Segmentation Mask and with "Create an Image" tool. Than I used FiberApp and it worked quite ok.Ana
thank you for explaining the concept in details. I tried your macro and it works well. Still I want to repeat the steps by myself and I'm not sure that I understand the intensity calibration completely(point number 5)? Still, this is really usefull. A big thanks!
Another question.. I was trying to establish macro, which includes fiber separation.
The problem is that macro stops when it comes to FiberApp. Is it even possible to incorporate FiberApp in my macro?
Maybe is this possible with batch processing? I tried to add FiberApp to `Batch proccesing - Run after` but there are to many possibilities within Module `Fiber separation` and I'm not really sure which to choose. Best regards,
Public Function LoopOnSeparateFibers() As SimpleScript
LoopOnSeparateFibers = New SimpleScript
.MacroFile = "FiberSeparation>ModuleFiberSeparation.vb"
.MacroName = "SeparateFibers"
are there maybe some tutorials or guidlines for working in "code mode"? I have been working with macros and apps for a while, but I get stuck when it comes to codes. For example I'm not sure where to insert macro from previous post, that I can use it in Batch processing as a loop. I know that this is probably basic stuff, but I am completly new to this and I would need some guidlines. Thank you. Best regards, Ana