Image Pro Plus to read an ELISA Plate

mneurotech

Used the Mean Intensity function with ROI's for each well of a 96 well plate.  Free software online allowed for fitting of '1 / Intensity,Mean' values to a 4-parameter fit to create the standard curve from the standard wells, and subsequent quantification of sample wells.  The ELISA was an colorimetric HRP conjugation.  Comparison to actual data read by our Microplate reader was within 30%.  Curious if anyone else has tried this, and if there may be an even better Image Pro function to quantitate the well color.  I also tried Intensity - Blue, Green, Red, Max, Uncalibrated; and Integrated OD, but the Mean Intensity was the best.