Measuring sample area
Hello. I am a new user of image-pro plus. I work with the histology samples and my project involves measuring sample area (entire sample). I measure sample area manually and it takes forever..., just wondering maybe there is some automatic features that I can use. Thank you in advance for your time. Look forward for some ideas 
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Best Answer
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2016-11-28-103440
Shebar --
The SCREEN CAPTURE is a composite of multiple SCREEN CAPTURES and I accidentally generated an image of the COUNT / SIZE DIALOG BOX with both the BRIGHT and DARK RADIO BUTTONS ENABLED. That should just be the BRIGHT OPTION. I apologize for the confusion this caused.
The steps / settings that create UNTITLED11 from B.TIF MASK are
COUNT / SIZE +
AUTOMATIC BRIGHT THRESHOLD
APPLY FILTER RANGES
OPTIONS +
AS SHOWN
SELECT MEASUREMENTS +
AS SHOWN
COUNT BUTTON
IMAGE MENU +
MAKE MASK
After you do this, you should have a "clean" image of your TISSUE AREA.
You can extract your TISSUE AREA MEASUREMENT from your equivalent of the B.TIF MASK image but sometimes it is nice to clean up the mask.
I hope this information is helpful.
-- Matt
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Answers
I think we (the FORUM) need to know a bit more about your challenge to advise you properly.
If you can provide a SAMPLE IMAGE or a SAMPLE IMAGE SET that illustrates your challenge, I'm sure that we would be able to help you. I believe that the FORUM will accept JPG IMAGES and TIF IMAGES.
Also, if you have information about the CALIBRATION for your SAMPLE IMAGE(S) (ex. um/pix), that would help us advise you.
I hope this information is helpful.
-- Matt
The SAMPLE.PNG image that you posted is very low resolution but I have used it to illustrate one of the ways to address your imaging challenge.
Attached, please find
2016-11-21-161826.jpg
In this SCREEN CAPTURE / ILLUSTRATION image:
A = ORIGINAL IMAGE that is SMART THRESHOLDED to obtain IMAGE B
B = MASK of SMART THRESHOLD applied to IMAGE A
C = ORIGINAL IMAGE that is MANUAL MEASURED to obtain IMAGE D
D = MASK of MANUAL AREA obtained in IMAGE C
E = .5 * IMAGE A
F = E + D
G = DUAL THRESHOLD of IMAGE F
Results in EXCEL (far right box) can be achieved with:
1) IMAGE B (GREEN OVAL) and IMAGE D (GREEN OVAL)
2) IMAGE G (GREEN OVALS)
I hope this information is helpful.
-- Matt
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I apologize . . . I did not notice that this question was relating to IMAGE-PRO PLUS and not IMAGE-PRO PREMIER.
The only feature that I was suggesting that PREMIER has that PLUS does not is the SMART THRESHOLD.
I have created another FLOW CHART type image that shows a PROCEDURE that will take your ORIGINAL IMAGE and turn it into a TISSUE AREA MEASUREMENT in IMAGE-PRO PLUS.
The other parts of the PROCEDURE shown yesterday are supported by FUNCTIONS and FEATURES within IMAGE-PRO PLUS.
I hope this information is helpful.
-- Matt
Super.
If these steps result in the analysis you need, they can be automated with an IMAGE-PRO PLUS MACRO so that they can be executed quickly and with very little user input.
-- Matt
Certainly.
CALIBRATE your IMAGES using the features in
IMAGE-PRO PLUS + MEASURE (MENU) + CALIBRATION + SPATIAL
and then any measurement extracted from the image will be calibrated (if it is appropriate).
-- Matt
Another question is when I select counting it doesn't give me a choice of both, how is showed in our picture (it gives either automatic bright objects or dark). How you would recommend to solve this problem?
When I do final counting area data is expressed in many numbers, to get the final area count, i need to sum the numbers. In your picture data is expressed as one parametre, so maybe I do something wrong. Maybe have some ideas? Thank you in advance for your help.