count stained particles
Hello,
I just start using the Image pro premier 9.2.
I want to count the stained particles in tissue slides but I'm a little bit confused how to get the results.
At first, I use smart segmentation and count/size tools but the result that I got seems to have lots of errors. Then, I try to use the threshold tool (by using multiple ranges of intensity and hue) and count. However, I don't know how to get the output in both count number and intensity (pixels). Could you please give me some suggestions or solutions about this?
Moreover, does image pro premier have a positive pixel algorithm for granules/particles quantitative analysis?
Thank you.
I just start using the Image pro premier 9.2.
I want to count the stained particles in tissue slides but I'm a little bit confused how to get the results.
At first, I use smart segmentation and count/size tools but the result that I got seems to have lots of errors. Then, I try to use the threshold tool (by using multiple ranges of intensity and hue) and count. However, I don't know how to get the output in both count number and intensity (pixels). Could you please give me some suggestions or solutions about this?
Moreover, does image pro premier have a positive pixel algorithm for granules/particles quantitative analysis?
Thank you.
0
Answers
The "Smart Segmentation" tutorial can give you the info you want. The object intensity is reported by "Density (mean)" measurement. (also min, max, std.dev)
Yuri
I have watch some tutorials but I will check out more of them then.
I got the report like you said. However, Is it possible if i want to get customized measurement (like custom the range of intensity.)?
Thanks.