You can use line profile, mouse cursor (intensity is reported in the status bar) and if you segment the objects, the brightness is reported as Den./Inten.(mean) in the measurements table.
You can use line profile, mouse cursor (intensity is reported in the status bar) and if you segment the objects, the brightness is reported as Den./Inten.(mean) in the measurements table.
Thanks for your quick reply. I have a follow up question. How do I use the segmentation tool in conjunction with the line profile tool? The output's units in the measurements table is "intensity" in the y-axis and "distance (pixel)" in the x-axis, and I'm not sure how to interpret these units.
We are working with images of leaves. I am trying to correlate the presence of leaf hairs to image brightness, so that we can measure leaf hair count without actually counting all the leaf hairs (which would be tedious/impossible). We would like to do this by establishing an area of interest on the leaf and possibly comparing to a white image as a reference/baseline value. I've determined how to establish a reference value using the user manual, but I am still unsure how to interpret the results, or if this is even the best way to you about doing this.
Intensity is the measure of Gray level pixel values. There is Intensity calibration available to convert gray levels to appropriate units. Though reading your further comments I think that you don't really need any intensity measurements, you just want to segment the leaves and count hairs. This may involve pre-processing, image segmentation and count. You may look at our Media Library to see what tools are available in Premier: http://www.mediacy.com/resources/media
You can also post a sample image on the forum with detailed explanation what information you want to extract, so we have a better idea about your task.
I was unable to find images on the INTERNET that seemed to match the images that you are describing. As a substitute I found a picture of DONUTS with slightly varying amounts of SPRINKLES.
I have carried this picture through a process that analyzes the image and generates raw data that could be used for a SPRINKLE INDEX.
I have done and attached some SCREEN CAPTURES that I believe illustrate the type of process that could be used to generate a HAIR INDEX for your leaves.
I hope this information is helpful.
-- MATT
A = ORIGINAL IMAGE + SMART THRESHOLD + COUNT B = MASK OF A C = MASK OF HOLES from A using ROIs from B D = B - C
E = A + MEASUREMENTS created using boundaries found with BRIGHT THRESHOLD on D
F = SAME as E DATA TABLE on F shows HETEROGENEITY and INTENSITY STD DEV which could individually or combined be used for a SPRINKLE (HAIR) INDEX. F SORTED = F + COUNT SIZE RIBBON + IMAGE SECTION + SORT
Based on this, the MOST SPRINKLED DONUT is the P1R3 and the LEAST SPRINKLED DONUT is P1R2 or P1R4.
Answers
You may also check various tutorials in our media library http://www.mediacy.com/resources/media
Yuri
Thanks for your quick reply. I have a follow up question. How do I use the segmentation tool in conjunction with the line profile tool? The output's units in the measurements table is "intensity" in the y-axis and "distance (pixel)" in the x-axis, and I'm not sure how to interpret these units.
We are working with images of leaves. I am trying to correlate the presence of leaf hairs to image brightness, so that we can measure leaf hair count without actually counting all the leaf hairs (which would be tedious/impossible). We would like to do this by establishing an area of interest on the leaf and possibly comparing to a white image as a reference/baseline value. I've determined how to establish a reference value using the user manual, but I am still unsure how to interpret the results, or if this is even the best way to you about doing this.
Thank you for reading.
You may look at our Media Library to see what tools are available in Premier: http://www.mediacy.com/resources/media
You can also post a sample image on the forum with detailed explanation what information you want to extract, so we have a better idea about your task.
Yuri
KTGERLOFF --
I was unable to find images on the INTERNET that seemed to match the images that you are describing. As a substitute I found a picture of DONUTS with slightly varying amounts of SPRINKLES.
I have carried this picture through a process that analyzes the image and generates raw data that could be used for a SPRINKLE INDEX.
I have done and attached some SCREEN CAPTURES that I believe illustrate the type of process that could be used to generate a HAIR INDEX for your leaves.
I hope this information is helpful.
-- MATT
A = ORIGINAL IMAGE + SMART THRESHOLD + COUNT
B = MASK OF A
C = MASK OF HOLES from A using ROIs from B
D = B - C
E = A + MEASUREMENTS created using boundaries found with BRIGHT THRESHOLD on D
F = SAME as E
DATA TABLE on F shows HETEROGENEITY and INTENSITY STD DEV which could individually or combined be used for a SPRINKLE (HAIR) INDEX.
F SORTED = F + COUNT SIZE RIBBON + IMAGE SECTION + SORT
Based on this, the MOST SPRINKLED DONUT is the P1R3 and the LEAST SPRINKLED DONUT is P1R2 or P1R4.