Section clear remaining part blurred
I am trying to take a picture of circular intestinal organoid. If I focus on periphery, the center part gets blurred and vice-versa. Is there any way to get whole section focussed. Thank you.
It looks like you've found HDR tool, but you need EDF (in the same ribbon group).
Image-Pro has EDF (Extended Depth of Field) tool that purpose. You can capture multiple images with different Z positions and then combine all of them in EDF tool creating Best-Focus image.
In the same problem, will use of
1. filters or
2. HDR merge, global tone mapping
Will help any better.
Also, I took section image (only central part) using Zoom. Sharpness decreased. Will there be any option to sharpness while focusing only small central section.