smart count
I'm trying to count the cells using "Smart" in Count/Size tap. however, the attached or nearby cells are not recognized separately.(please, refer to the following image). What should I do? 
Raw image

Raw image

0
Answers
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Minhui,
Try to activate "Split with Count" option in the Split button dropdown. You can choose Watershed or Boundary shape option.
Yuri0 -
Hello YuriG!
Thank for your reply.
however, I already tried "Split with count" and It is hard to separate the attached cells completely(Some were not separated.)
Is there any other option?
Thank you!0 -
Hi Minhui,
I can see that your cells have a bright area (a couple of pixels) in the center. You can use Top-Hat filter (3D filters, Morpho group) to detect these areas as pre-processing step, then you can much easier segment them separately.
Yuri
0 -
Hi Minhui,
I've attached several options that use Top-Hat filter in smart segmentation to count the cells on your image.
The screenshot below shows the result of ringtophatgrow.iqo (load the image, load the IQO file from Count/Size tab, Measurements group and click Count):
Yuri0
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