Are we able to measure across surface plot to measure grooves? Or display surface plot in um instead

Serene
Serene
in Image-Pro General Discussions
I want to measure the depth of my groove but i cannot measure across the plot. 
Line profile also only provides distance and not depth. 
Or anyone knows how to change legend of surface plot to um/nm instead of intensity?

Best Answer

  • YuriG
    YuriG
    Answer ✓
    Hi Serene,

    Regarding if the intensity is real depth: it depends on how the images were acquired. If you used an Atomic Force Microscope (AMF) where intensity of pixels define the depth or you created a Topo map from a through-focus sequence using the EDF tool, then the pixel values is the real depth. But if you just used a bright-field image, then no, intensity doesn't correspond to depth, it's just pixel brightness that is defined by illumination and material properties.

    Yuri

Answers

  • YuriG
    YuriG
    edited August 2024
    Hi Serene,

    If the depth is represented by intensity on your images, you can use Intensity calibration to measure the depth. (Capture tab, Calibration options dropdown). Create a new intensity calibration and set units to µm. The surface plot and line profile will show the depth in calibrated units:



    You can also show the pixel values in calibrated units in the status bar by activating this option:
     
    Regards,

    Yuri
  • Serene
    Serene
    Hi Yuri, 

    Thank you for the response! 
    But I still cannot get the depth in um. Can you help to see what i did wrong? 




  • YuriG
    YuriG
    Hi Serene,

    I don't see anything what could be wrong on your screenshot. The units should be shown on Z axis. Do you see units on Line profile graph? Maybe it's a refresh issue. 
    Try applying the intensity calibration and then showing the surface plot.

    Yuri
  • Serene
    Serene
    Hi Yuri, 

    I closed it and refreshed the surface plot but it still does not reflect the unit. 
    The line profile does show though. 

    But is the intensity threshold = real depth in um? or it's just the unit being shown? 
    Kindly help. 

    Serene

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