valpal
URGENT Question regarding Percent of Area%. I need to measure the amount of positive (DAB positive) in IHC pictures. After I assign class positive and class negative (the nuclei), I need to select background in order to count the amount of negative and positive pixels. How is the percent of area calculated? what it is Area (pixel^2)? I need to understand how the background area is influencing the percent of positive and how should I take into account the negative area for the analysis. thanks please let me know as soon as possible