Attempting to use the alignment tool- AutoQuant X 3.1.2

microscopyra

Hi!  Not sure if this is the correct place for this kind of question, but if not, please point me in the appropriate direction!
I have been attempting to run an alignment on a 2 color fluorescent Z stack where the mouse jumped a bit during one of the frames (CZI file, LSM880).
Processing->Image Alignment->Slice to Slice
The process crashes within 5 seconds every time with an error similar to this one:

Problem signature:

  Problem Event Name: CLR20r3

  Problem Signature 01: aqiPlatform.exe

  Problem Signature 02: 3.1.2.0

  Problem Signature 03: 59692193

  Problem Signature 04: AutoAlignOperations

  Problem Signature 05: 1.0.0.1

  Problem Signature 06: 596924ce

  Problem Signature 07: 24

  Problem Signature 08: cd

  Problem Signature 09: System.AccessViolationException

  OS Version: 6.1.7601.2.1.0.256.4

  Locale ID: 1033

  Additional Information 1: 280d

  Additional Information 2: 280d17e932c988d6f296e811a53ae5a1

  Additional Information 3: 0945

  Additional Information 4: 0945bbae75119a17c3551191358bf4b6

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This has happened whether I use recommended settings or attempt to adjust the settings (Black replacement, higher noise thresholds, Severe warping, etc).  In the User settings, both temporary files and the default image folder have been set to fully accessible "temp" folders in the base C drive.

Is there anything else I can try, or has anyone run into this problem before?  Please let me know if there is any additional information I can provide.

AndyM

That is indeed what I was looking for, though the problem seems like it may be more data-specific than the dimensions alone. I will send a message to you directly for additional information.

AndyM

This is a perfectly fine place to ask. I don't believe we've seen that one before, so I'll need to ask a question or two that will help us track down what happened. For starters, what are the XYZ dimensions of your stack?

microscopyra

Is this enough to go off of?